C（3〜6か月） Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl] Developed by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true Cmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression. C (3-6 months) 小堤　保則 E14 [129P2/OlaHsd] Cmah null mouse, KZO Cmah null mouse, KZO B6.129P2-Cmah<tm1Ykoz> B6.129P2-Cmah<tm1Ykoz> Kyoto Univ. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 27, 3008-3022 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use of the BIOLOGICAL RESOURCE only for publication of research papers. 京都大学大学院生命科学研究科・竹松　弘、小堤保則（2000）。E14 ES細胞を用いて作出。C57BL/6背景。 Cmah遺伝子のノックアウトマウス。エクソン2にneoカセットが挿入されている。ホモマウスはB細胞活性化亢進が見らる。行動異常を呈し、繁殖成績は低い。 国立大学法人京都大学 Yasunori KOZUTSUMI 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Mol. Cell. Biol., 27, 3008-3022 (2007).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>公表を前提とした学術研究に限る。 RBRC02322 Metabolism Research mouse PGK promoter, E.coli reomycin resistance gene, mouse Cmah genomic DNA <a href='https://brc.riken.jp/mus/pcr02322'>Genotyping protocol -PCR-</a>